Fluorescence in situ Hybridization (FISH) - Tumour (Fresh/FFPE)

Alternate test name

Tumour FISH tests include the following (testing will be based on indication):

Lymphoma: Burkitt - MYC, IGH/MYC

Lyphoma: Anaplastic Large Cell Lymphoma - ALK

Other Lymphoma - IR4/DUSP22, BCL6, IGH/BCL2, IGH/CCND1, TCRa/d

Alveolar Rhabdomyosarcoma - FOXO1

Aneurysmal bone cyst - USP6

Dermatofibrosarcoma protuberans - PDGFB

Ewing’s tumours - EWSR1

Infantile fibrosarcoma - TEL(ETV6)

Liposarcoma - CHOP, FUS

Nodular fasciitis - USP6

NUTM1-rearranged neoplasia - NUTM1

RCC/ASPS/PEComa - TFE3

Retinoblastoma - RB1

Rhabdoid - SMARCB1

Synovial sarcoma - SYT

Undifferentiated sarcoma - CIC

Nervous system tumours

Astrocytoma - BRAF

Ependymoma - RELA

Medulloblastoma - NMYC, CMYC, MYB/Centromere 6

Neuroblastoma - NMYC, 1p36

Oligodendroglioma - 1p36/1q25 & 19q13/19p13

PNET - C19MC

Other solid tumour - CDKN2A, PTEN, ATM, TP53, Whole chromosome paints, Chromosome enumeration

Lab area

Genome Diagnostics - Cytogenetics

Method and equipment

Direct preparation and culture; FISH (Fluorescence in situ hybridization)

FFPE (Paraffin) FISH (Fluorescence in situ hybridization)

Expected turn-around time

2-5 days (fresh tissue); 2 weeks (FFPE tissue)

Specimen type

Tumour

Specimen requirements

Minimum 2-3 mm³ fresh tissue (fresh biopsy samples)

FFPE - minimum of two 4 micron slides

Storage and transportation

Fresh tumour - refrigerate until time of shipment. Ship sample overnight at room temperature for receipt at SickKids Cytogenetics Laboratory within 24 hours of collection.

Paraffin Sections - Minimum of two 4 micron slides, room temperature. Include a circled H&E slide if the tumour is in a limited area of the tissue section.

Special requirements

Fresh tumour - Place in sterile container with SickKids Cytogenetics transport media or other sterile culture media.

Shipping information

The Hospital for Sick Children

Division of Genome Diagnostics

555 University Avenue, Black Wing, Room 3416

Toronto, ON

Canada

M5G 1X8

Phone: 416-813-7200 ext. 2

Hours: Monday to Friday, 8 a.m. to 4:30 p.m.

Off hours: Please send to Rapid Response Laboratory, 555 University Avenue, Room 3642